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Cadmium and wild boar: Environmental exposure and immunological impact on macrophages.

ABSTRACT: Cadmium (Cd²⁺) is regarded as one of the most toxic heavy metals, which can enter the food chain through environmental contamination and be bioaccumulated. Its exposure in Ligurian wild boars was monitored between 2016-2020 and revealed high level of this heavy metal in different provinces. In one of these polluted area, 21 wild boars were additionally sampled and the relationship between hepatic and renal Cd²⁺ concentration suggested that majority of these animals presented chronic intoxication. Cd²⁺ exposure of wild boar might lead to an immunosuppression status, thus in vitro experiments on wild boar monocyte-derived macrophages (moMФ) were carried out. Effects of Cd²⁺ scalar doses were evaluated through viability and adsorption assays, ELISA, qPCR. Moderate doses of this environmental pollutant (20 μM) were absorbed by moMФ, with subsequent reduction of their viability. This heavy metal did not trigger release of either IFN- β, anti-inflammatory or pro-inflammatory cytokines by moMФ, instead 24 h treatment with 20 μM of Cd²⁺ resulted in down-regulated expression of TNF-α, IL-12p40, several TLRs, CD14, MD2, BD2, MyD88, p65, and NOS2. The results of our monitoring activity suggested that wild boar can be useful to monitor environmental exposure of this heavy metal and can help in understanding the type of contamination. In addition, in vitro experiments on wild boar moMФ revealed that Cd²⁺ exposure negatively affected the immune function of these cells, likely leading to increased susceptibility to infection.

SUBMITTER: Franzoni G

PROVIDER: S-EPMC8819190 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Cadmium and wild boar: Environmental exposure and immunological impact on macrophages.

Franzoni Giulia G Ciccotelli Valentina V Masiello Lucia L De Ciucis Chiara Grazia CG Anfossi Antonio Giovanni AG Vivaldi Barbara B Ledda Mauro M Zinellu Susanna S Dei Giudici Silvia S Berio Enrica E Tiziana Andreoli A Dellepiane Monica M Zoppi Simona S Masotti Chiara C Crescio Maria Ines MI Oggiano Annalisa A Ercolini Carlo C Razzuoli Elisabetta E

Toxicology reports 20220129

Cadmium (Cd<sup>2+</sup>) is regarded as one of the most toxic heavy metals, which can enter the food chain through environmental contamination and be bioaccumulated. Its exposure in Ligurian wild boars was monitored between 2016-2020 and revealed high level of this heavy metal in different provinces. In one of these polluted area, 21 wild boars were additionally sampled and the relationship between hepatic and renal Cd<sup>2+</sup> concentration suggested that majority of these animals presente ...[more]

PMID: 35145881

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Project description:BackgroundThe application of metabolomics-based profiles in environmental epidemiological studies is a promising approach to refine the process of health risk assessment. We aimed to identify potential metabolomics-based profiles in urine and plasma for the detection of relatively low-level cadmium (Cd) exposure in large population-based studies.MethodWe analyzed 123 urinary metabolites and 94 plasma metabolites detected in fasting urine and plasma samples collected from 1,412 men and 2,022 women involved in the Tsuruoka Metabolomics Cohort Study. Regression analysis was performed for urinary N-acetyl-beta-D-glucosaminidase (NAG), plasma, and urinary metabolites as dependent variables, and urinary Cd (U-Cd, quartile) as an independent variable. The multivariable regression model included age, gender, systolic blood pressure, smoking, rice intake, BMI, glycated hemoglobin, low-density lipoprotein cholesterol, alcohol consumption, physical activity, educational history, dietary energy intake, urinary Na/K ratio, and uric acid. Pathway-network analysis was carried out to visualize the metabolite networks linked to Cd exposure.ResultUrinary NAG was positively associated with U-Cd, but not at lower concentrations (Q2). Among urinary metabolites in the total population, 45 metabolites showed associations with U-Cd in the unadjusted and adjusted models after adjusting for the multiplicity of comparison with FDR. There were 12 urinary metabolites which showed consistent associations between Cd exposure from Q2 to Q4. Among plasma metabolites, six cations and one anion were positively associated with U-Cd, whereas alanine, creatinine, and isoleucine were negatively associated with U-Cd. Our results were robust by statistical adjustment of various confounders. Pathway-network analysis revealed metabolites and upstream regulator changes associated with mitochondria (ACACB, UCP2, and metabolites related to the TCA cycle).ConclusionThese results suggested that U-Cd was associated with metabolites related to upstream mitochondrial dysfunction in a dose-dependent manner. Our data will help develop environmental Cd exposure profiles for human populations.

Dataset Information

Cadmium and wild boar: Environmental exposure and immunological impact on macrophages.

Publications

Cadmium and wild boar: Environmental exposure and immunological impact on macrophages.

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