Protocol for influenza A virus infection of mice and viral load determination.
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ABSTRACT:
SUBMITTER: Galani IE
PROVIDER: S-EPMC8819391 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
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Protocol for influenza A virus infection of mice and viral load determination.
Galani Ioanna-Evdokia IE Triantafyllia Vasiliki V Eleminiadou Evridiki-Evangelia EE Andreakos Evangelos E
STAR protocols 20220203 1
Influenza A viruses (IAVs) are common respiratory viruses. Mouse models of IAV infection are valuable to study the mechanisms of IAV infection and pathology. Here, we present a detailed protocol for IAV infection of mice via intranasal administration. We detail the processing of mouse lung tissue and then describe the determination of viral load by several approaches including RNA, protein, or plaque-forming unit assays. This protocol may be adapted to other influenza strains or respiratory viru ...[more]
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