Developmental alterations in DNA methylation during gametogenesis from primordial germ cells to sperm.

ABSTRACT: Because epigenetics is a critical component for gene expression, the hypothesis was tested that DNA methylation alterations are dynamic and continually change throughout gametogenesis to generate the mature sperm. Developmental alterations and stage-specific DNA methylation during gametogenesis from primordial germ cells (PGCs) to mature sperm are investigated. Individual developmental stage germ cells were isolated and analyzed for differential DNA methylation regions (DMRs). The number of DMRs was highest in the first three comparisons with mature PGCs, prospermatogonia, and spermatogonia. The most statistically significant DMRs were present at all stages of development and had variations involving both increases or decreases in DNA methylation. DMR-associated genes were identified and c

SUBMITTER: Ben Maamar M 

PROVIDER: S-EPMC8819394 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature