Single-cell transcriptomics reveals a low CD8⁺ T cell infiltrating state mediated by fibroblasts in recurrent renal cell carcinoma.

ABSTRACT:

Purpose

Recurrent renal cell carcinoma(reRCC) is associated with poor prognosis and the underlying mechanism is not yet clear. A comprehensive understanding of tumor microenvironment (TME) of reRCC may aid in designing effective anticancer therapies, including immunotherapies. Single-cell transcriptomics holds great promise for investigating the TME, however, this technique has not been used in reRCC. Here, we aimed to explore the difference in the TME and gene expression pattern between primary RCC (pRCC) and reRCC at single-cell level.

Experimental design

We performed single-cell RNA sequencing analyses of 32,073 cells from 2 pRCC, 2 reRCC, and 3 adjacent normal kidney samples. 41 pairs of pRCC and reRCC samples were collected as a validation cohort to assess differences o

SUBMITTER: Peng YL 

PROVIDER: S-EPMC8819783 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature