Transgenic rice Oryza glaberrima with higher CPD photolyase activity alleviates UVB-caused growth inhibition.

ABSTRACT: The ultraviolet B (UVB) sensitivity of rice cultivated in Asia and Africa varies greatly, with African rice cultivars (Oryza glaberrima Steud. and O. barthii A. Chev.) being more sensitive to UVB because of their low cyclobutane pyrimidine dimer (CPD) photolyase activity, which is a CPD repair enzyme, relative to Asian rice cultivars (O. sativa L.). Hence, the production of UVB-resistant African rice with augmented CPD photolyase activity is of great importance, although difficulty in transforming the African rice cultivars to this end has been reported. Here, we successfully produced overexpressing transgenic African rice with higher CPD photolyase activity by modifying media conditions for callus induction and regeneration using the parental line (PL), UVB-sensitive

SUBMITTER: Mmbando GS 

PROVIDER: S-EPMC8820246 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature