Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response.

ABSTRACT: Under starvation conditions, bacteria tend to slow down their translation rate by reducing rRNA synthesis, but the way they accomplish that may vary in different bacteria. In Mycobacterium species, transcription of rRNA is activated by the RNA polymerase (RNAP) accessory transcription factor CarD, which interacts directly with RNAP to stabilize the RNAP-promoter open complex formed on rRNA genes. The functions of CarD have been extensively studied, but the mechanisms that control its expression remain obscure. Here, we report that the level of CarD was tightly regulated when mycobacterial cells switched from nutrient-rich to nutrient-deprived conditions. At the translational level, an antisense RNA of carD (AscarD) was induced in a SigF-dependent manner to bind with carD</

SUBMITTER: Li X 

PROVIDER: S-EPMC8820732 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature