Picturing of the Lung Tumor Cellular Composition by Multispectral Flow Cytometry.

ABSTRACT: The lung tumor microenvironment plays a critical role in the tumorigenesis and metastasis of lung cancer, resulting from the crosstalk between cancer cells and microenvironmental cells. Therefore, comprehensive identification and characterization of cell populations in the complex lung structure is crucial for development of novel targeted anti-cancer therapies. Here, a hierarchical clustering approach with multispectral flow cytometry was established to delineate the cellular landscape of murine lungs under steady-state and cancer conditions. Fluorochromes were used multiple times to be able to measure 24 cell surface markers with only 13 detectors, yielding a broad picture for whole-lung phenotyping. Primary and metastatic murine lung tumor models were included to detect major cell popul

SUBMITTER: Olesch C 

PROVIDER: S-EPMC8821098 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature