Real-time imaging of asthmatic epithelial cells identifies migratory deficiencies under type-2 conditions.

ABSTRACT:

Background

The epithelium is increasingly recognized as a pathologic contributor to asthma and its phenotypes. Although delayed wound closure by asthmatic epithelial cells is consistently observed, underlying mechanisms remain poorly understood, partly due to difficulties in studying dynamic physiologic processes involving polarized multilayered cell systems. Although type-2 immunity has been suggested to play a role, the mechanisms by which repair is diminished are unclear.

Objectives

This study sought to develop and utilize primary multilayered polarized epithelial cell systems, derived from patients with asthma, to evaluate cell migration in response to wounding under type-2 and untreated conditions.

Methods

A novel wounding device for multilayered polarized cells,

SUBMITTER: Jin M 

PROVIDER: S-EPMC8821171 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature