Calibr improves spectral library search for spectrum-centric analysis of data independent acquisition proteomics.

ABSTRACT: Identifying peptides and proteins from mass spectrometry (MS) data, spectral library searching has emerged as a complementary approach to the conventional database searching. However, for the spectrum-centric analysis of data-independent acquisition (DIA) data, spectral library searching has not been widely exploited because existing spectral library search tools are mainly designed and optimized for the analysis of data-dependent acquisition (DDA) data. We present Calibr, a spectral library search tool for spectrum-centric DIA data analysis. Calibr optimizes spectrum preprocessing for pseudo MS2 spectra, generating an 8.11% increase in spectrum-spectrum match (SSM) number and a 7.49% increase in peptide number over the traditional preprocessing approach. When searching against the DDA-bas

SUBMITTER: Wang JH 

PROVIDER: S-EPMC8821666 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature