Construction and Immunogenicity of a Recombinant Pseudorabies Virus Variant With TK/gI/gE/11k/28k Deletion.

ABSTRACT: In China, the re-emerging pseudorabies virus (PRV) variant has caused large-scale outbreaks of pseudorabies in swine herds with classical PRV vaccine immunization since late 2011. Here, a recombinant PRV with TK/gI/gE/11k/28k deletion was constructed based on variant HN1201 strain isolated in 2012, by the bacterial artificial chromosome infectious clones. Compared with the parental virus, the recombinant PRV rHN1201^{TK-/gE-/gI-/11k-/28k-} showed a similar virus grown curve and exhibited smaller plaques. The vaccination of rHN1201^{TK-/gE-/gI-/11k-/28k-} could elicit an earlier and higher level of gB antibody, and the neutralizing antibodies elicited by rHN1201^{TK-/gE-/gI-/11k-/28k-}

SUBMITTER: Yan S 

PROVIDER: S-EPMC8821880 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature