A broad and systematic approach to identify B cell malignancy-targeting TCRs for multi-antigen-based T cell therapy.

ABSTRACT: CAR T cell therapy has shown great promise for the treatment of B cell malignancies. However, antigen-negative escape variants often cause disease relapse, necessitating the development of multi-antigen-targeting approaches. We propose that a T cell receptor (TCR)-based strategy would increase the number of potential antigenic targets, as peptides from both intracellular and extracellular proteins can be recognized. Here, we aimed to isolate a broad range of promising TCRs targeting multiple antigens for treatment of B cell malignancies. As a first step, 28 target genes for B cell malignancies were selected based on gene expression profiles. Twenty target peptides presented in human leukocyte antigen (HLA)-A∗01:01, -A∗24:02, -B∗08:01, or -B∗35:01 were identified from the immunopeptidome of

SUBMITTER: Meeuwsen MH 

PROVIDER: S-EPMC8821929 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature