Genome-Wide Identification of Genes Important for Growth of <i>Dickeya dadantii</i> and <i>Dickeya dianthicola</i> in Potato (<i>Solanum tuberosum</i>) Tubers.

ABSTRACT: Dickeya species are causal agents of soft rot diseases in many economically important crops, including soft rot disease of potato (Solanum tuberosum). Using random barcode transposon-site sequencing (RB-TnSeq), we generated genome-wide mutant fitness profiles of Dickeya dadantii 3937, Dickeya dianthicola ME23, and Dickeya dianthicola 67-19 isolates collected after passage through several in vitro and in vivo conditions. Though all three strains are pathogenic on potato, D. dadantii 3937 is a well-characterized model while D. dianthicola strains ME23 and 67-19 are recent isolates. Strain ME23 specifically was identified as a representative strain from a 2014 outbreak on potato. This study generated comparable gene fitness measur

SUBMITTER: Helmann TC 

PROVIDER: S-EPMC8821946 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature