Proteasome Activator Blm10 Regulates Transcription Especially During Aging.

ABSTRACT:

Background

Histones are basic elements of the chromatin and are critical to controlling chromatin structure and transcription. The proteasome activator PA200 promotes the acetylation-dependent proteasomal degradation of the core histones during spermatogenesis, DNA repair, transcription, and cellular aging and maintains the stability of histone marks.

Objective

The study aimed to explore whether the yeast ortholog of PA200, Blm10, promotes degradation of the core histones during transcription and regulates transcription especially during aging.

Methods

Protein degradation assays were performed to detect the role of Blm10 in histone degradation during transcription. mRNA profiles were compared in WT and mutant BY4741 or MDY510 yeast cells by RNA-sequencing.

Results<

SUBMITTER: Chen YS 

PROVIDER: S-EPMC8822228 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature