Effects of mechanical stimulation on metabolomic profiles of SW1353 chondrocytes: shear and compression.

ABSTRACT: Mechanotransduction is a biological phenomenon where mechanical stimuli are converted to biochemical responses. A model system for studying mechanotransduction are the chondrocytes of articular cartilage. Breakdown of this tissue results in decreased mobility, increased pain, and reduced quality of life. Either disuse or overloading can disrupt cartilage homeostasis, but physiological cyclical loading promotes cartilage homeostasis. To model this, we exposed SW1353 cells to cyclical mechanical stimuli, shear and compression, for different durations of time (15 and 30 min). By utilizing liquid chromatography-mass spectroscopy (LC-MS), metabolomic profiles were generated detailing metabolite features and biological pathways that are altered in response to mechanical stimulation. In total, 14

SUBMITTER: Welhaven HD 

PROVIDER: S-EPMC8822358 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature