DsRNA Induced IFNβ-MMP13 Axis Drives Corneal Wound Healing.

ABSTRACT:

Purpose

Cornea, the outermost transparent layer of the eye, is the first line of defense against external threats. Following injury, the wound healing response is crucial to corneal repair and regeneration, yet its underlying mechanism is poorly understood. Our study was designed to investigate the role of dsRNA and its regulatory network in corneal wound healing.

Methods

A corneal wound healing model was established via the surgical removal of half of the corneal surface and adjoining limbus. RNase III was then used to clarify the role of dsRNA in corneal wound closure and RNA-seq was performed to investigate the mechanism of dsRNA in the healing process. Related gene expression was assessed using immunofluorescence staining, qPCR, and Western blot. Flow cytometry and scrat

SUBMITTER: Lan X 

PROVIDER: S-EPMC8822365 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature