Dataset Information

The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies.

ABSTRACT: Human milk (HM) lipidome stability during storage is crucial in lipidomic studies to avoid misinterpretations. Facing the lack of comprehensive work on the HM lipidome stability, we performed a study on a potential alteration in the lipid profiles of HM samples stored under different conditions. An untargeted LC-Q-TOF-MS-based approach was applied to study the influence of storage conditions as well as the interaction of the storage temperature and time on HM lipid profiles. The samples were stored for 4-84 days at temperatures in the range from 4 to -80 °C and also were exposed to up to three freeze-thaw cycles. The results showed that the storage at 4 °C for just 4 days as well as being subjected to three freeze-thaw cycles can lead to a change in the content of lipids. The observed differences in levels of some lipid species in samples stored at -20 °C in comparison to the concentration level of those lipids in samples stored at -80 °C were not statistically significant, and inter-individual variance regardless of sample storage condition was maintained. The storage of HM samples at -20 °C for up to 3 weeks and -80 °C for up to 12 weeks ensures sample lipidome stability.

SUBMITTER: Garwolinska D

PROVIDER: S-EPMC8822481 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies.

Garwolińska Dorota D Młynarczyk Michał M Kot-Wasik Agata A Hewelt-Belka Weronika W

Journal of proteome research 20211229 2

Human milk (HM) lipidome stability during storage is crucial in lipidomic studies to avoid misinterpretations. Facing the lack of comprehensive work on the HM lipidome stability, we performed a study on a potential alteration in the lipid profiles of HM samples stored under different conditions. An untargeted LC-Q-TOF-MS-based approach was applied to study the influence of storage conditions as well as the interaction of the storage temperature and time on HM lipid profiles. The samples were sto ...[more]

PMID: 34965729

Similar Datasets

Project description:ObjectivesThis study aimed to assess the effects of storage temperature on the lipidomics profile change in Pacific saury (Cololabis saira).MethodsIn this paper, C. saira underwent frozen storage at two different temperatures, T1 (-18 °C) and T2 (-25 °C), for a duration of three months. Chemical and lipidomic methods were used to determine the changes in lipids during the storage process.ResultsResults showed that the content of triglyceride and phospholipid decreased significantly (p < 0.05), and free fatty acid increased significantly (p < 0.05), while the content of total cholesterol remained relatively constant across different storage temperatures. Additionally, an increasing trend in AV, POV, and TBARS contents was observed after the freezing process, with lipid oxidation being significantly higher in the -18 °C group compared to the -25 °C group (p < 0.05). A comprehensive analysis identified 4854 lipid molecules in the muscles of C. saira, categorized into 46 lipid subclasses, predominantly including triglycerides (TG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylglycerol (PG), and diglycerides (DG). Among them, TG was the most abundant lipid, followed by PC. Using orthogonal partial least squares discriminant analysis (OPLS-DA) with a variable importance in projection (VIP) score > 1 and p value < 0.05 as criteria, 338, 271, and 103 highly significantly differentiated lipids were detected in the comparison groups CK vs. T1, CK vs. T2, and T1 vs. T2, respectively. The results indicated that storage at -18 °C had a more pronounced effect than storage at -25 °C. During the freezing process, TG expression was significantly down-regulated, and TG(18:4_14:0_20:5), TG(20:5_13:0_22:6), TG(22:6_14:1_22:6), and TG(18:4_13:0_22:6) were the most predominant individuals. The CK group was initially present in C. saira before storage. Differential lipid molecules in the CK vs. T1 and CK vs. T2 groups were screened using a fold change (FC) > 2 or FC < 0.5. In the CK vs. T2 group, 102 highly significant differential lipid molecules were identified, with 55 being down-regulated across seven subclasses. In contrast, the CK vs. T1 group revealed 254 highly significant differential lipid molecules, with 85 down-regulated across 13 subclasses. The results showed that more PCs and PEs were down-regulated, with a higher differential abundance of PE and PC in the -25 °C group compared to the -18 °C group. The differential metabolites were primarily enriched in 17 metabolic pathways, with glycerophospholipid metabolism being the most prominent, followed by sphingolipid metabolism during the frozen storage.ConclusionsOverall, -25 °C storage in production was more favorable for maintaining the lipid stability of C. saira. This work could provide useful information for aquatic product processing and lipidomics.

Dataset Information

The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies.

Publications

The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets