Force measurements of Myosin II waves at the yolk surface during Drosophila dorsal closure.

ABSTRACT: The mechanical properties and the forces involved during tissue morphogenesis have been the focus of much research in the last years. Absolute values of forces during tissue closure events have not yet been measured. This is also true for a common force-producing mechanism involving Myosin II waves that results in pulsed cell surface contractions. Our patented magnetic tweezer, CAARMA, integrated into a spinning disk confocal microscope, provides a powerful explorative tool for quantitatively measuring forces during tissue morphogenesis. Here, we used this tool to quantify the in vivo force production of Myosin II waves that we observed at the dorsal surface of the yolk cell in stage 13 Drosophila melanogaster embryos. In addition to providing for the first time to our knowledge quantitati

SUBMITTER: Selvaggi L 

PROVIDER: S-EPMC8822616 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature