A simple immunoassay for extracellular vesicle liquid biopsy in microliters of non-processed plasma.

ABSTRACT:

Background

Extracellular vesicles (EVs), released by most cell types, provide an excellent source of biomarkers in biological fluids. However, in order to perform validation studies and screenings of patient samples, it is still necessary to develop general techniques permitting rapid handling of small amounts of biological samples from large numbers of donors.

Results

Here we describe a method that, using just a few microliters of patient's plasma, identifies tumour markers exposed on EVs. Studying physico-chemical properties of EVs in solution, we demonstrate that they behave as stable colloidal suspensions and therefore, in immunocapture assays, many of them are unable to interact with a stationary functionalised surface. Using flocculation methods, like those used to des

SUBMITTER: Campos-Silva C 

PROVIDER: S-EPMC8822649 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature