Bifunctional optogenetic switch for improving shikimic acid production in E. coli.

ABSTRACT:

Background

Biomass formation and product synthesis decoupling have been proven to be promising to increase the titer of desired value add products. Optogenetics provides a potential strategy to develop light-induced circuits that conditionally control metabolic flux redistribution for enhanced microbial production. However, the limited number of light-sensitive proteins available to date hinders the progress of light-controlled tools.

Results

To address these issues, two optogenetic systems (TPRS and TPAS) were constructed by reprogramming the widely used repressor TetR and protease TEVp to expand the current optogenetic toolkit. By merging the two systems, a bifunctional optogenetic switch was constructed to enable orthogonally regulated gene transcription and protein accum

SUBMITTER: Komera I 

PROVIDER: S-EPMC8822657 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature