Fresh and cryopreserved ovarian tissue from deceased young donors yields viable follicles.

ABSTRACT:

Objective

Ovarian tissue cryopreservation is one of the crucial options for fertility preservation. Transplantation of cryopreserved ovarian tissue was proven to restore ovarian endocrine function in patients with premature ovarian insufficiency. Ovaries from deceased donors potentially serve as an excellent and readily available tissue for the translational and basic research. In this study, we used ovaries obtained from 5 deceased donors aged 18-26 years, to evaluate the number and quality of ovarian follicles isolated before and after cryopreservation.

Design

Preclinical.

Setting

Academic biomedical research laboratory.

Patients

De-identified deceased human donors.

Interventions

Slow-freeze cryopreservation and thawing.

Main outcome measures

Fo

SUBMITTER: Fan Y 

PROVIDER: S-EPMC8823279 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature