Heterologous Expression of Active Dehalobacter Respiratory Reductive Dehalogenases in Escherichia coli.

ABSTRACT: Reductive dehalogenases (RDases) are a family of redox enzymes that are required for anaerobic organohalide respiration, a microbial process that is useful in bioremediation. Structural and mechanistic studies of these enzymes have been greatly impeded due to challenges in RDase heterologous expression, potentially because of their cobamide-dependence. There have been a few successful attempts at RDase production in unconventional heterologous hosts, but a robust method has yet to be developed. Here we outline a novel respiratory RDase expression system using Escherichia coli. The overexpression of E. coli's cobamide transport system, btu, and anaerobic expression conditions were found to be essential for production of active RDases from Dehalobacter-an obligate organohalide

SUBMITTER: Picott KJ 

PROVIDER: S-EPMC8824204 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature