Seasonal Dynamics of Methanotrophic Bacteria in a Boreal Oil Sands End Pit Lake.

ABSTRACT: Base Mine Lake (BML) is the first full-scale demonstration end pit lake for the oil sands mining industry in Canada. We examined aerobic methanotrophic bacteria over all seasons for 5 years in this dimictic lake. Methanotrophs comprised up to 58% of all bacterial reads in 16S rRNA gene amplicon sequencing analyses (median 2.8%), and up to 2.7 × 10⁴ cells mL^-1 of water (median 0.5 × 10³) based on qPCR of pmoA genes. Methanotrophic activity and populations in the lake water were highest during fall turnover and remained high through the winter ice-covered period into spring turnover. They declined during summer stratification, especially in the epilimnion. Three methanotroph genera (Methylobacter, Methylovulum, and Methyloparacoccus

SUBMITTER: Albakistani EA 

PROVIDER: S-EPMC8824210 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature