Structural analysis of temperature-dependent alternative splicing of HsfA2 pre-mRNA from tomato plants.

ABSTRACT: Temperature-dependent alternative splicing was recently demonstrated for intron 2 of the gene coding for heat shock factor HsfA2 of the tomato plant Solanum lycopersicum, but the molecular mechanism regulating the abundance of such temperature-dependent splice variants is still unknown. We report here on regulatory pre-mRNA structures that could function as regulators by controlling the use of splice sites in a temperature-dependent manner. We investigate pre-mRNA structures at the splice sites of intron 2 of the gene coding for HsfA2 from S. lycopersicum using NMR- and CD-spectroscopy as well as in-line probing. The pre-mRNA undergoes conformational changes between two different secondary structures at the 3' splice site of the intron in a temperature-dependent manner. Previ

SUBMITTER: Broft P 

PROVIDER: S-EPMC8824230 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature