Transcriptome Analysis of Pseudomonas aeruginosa Biofilm Infection in an <i>Ex Vivo</i> Pig Model of the Cystic Fibrosis Lung.

ABSTRACT: Pseudomonas aeruginosa is the predominant cause of chronic biofilm infections that form in the lungs of people with cystic fibrosis (CF). These infections are highly resistant to antibiotics and persist for years in the respiratory tract. One of the main research challenges is that current laboratory models do not accurately replicate key aspects of a P. aeruginosa biofilm infection, highlighted by previous RNA-sequencing studies. We compared the P. aeruginosa PA14 transcriptome in an ex vivo pig lung (EVPL) model of CF and a well-studied synthetic cystic fibrosis sputum medium (SCFM). P. aeruginosa was grown in the EVPL model for 1, 2, and 7 days, and in vitro in SCFM for 1 and 2 days. The RNA was extracted and sequenced at each time point. Our findings demonstrate that expr

SUBMITTER: Harrington NE 

PROVIDER: S-EPMC8824274 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature