Evaluation of Cytologic Sample Preparations for Compatibility With Nucleic Acid Analysis.

ABSTRACT:

Objectives

In this study, the influence of several key elements of the cytologic sample workflow on DNA and RNA content was evaluated.

Methods

The A549 cell line, patient-derived organoids, and pleural effusions were used to investigate the effect of (1) several collection media and delayed time to processing; (2) cytology specimens; (3) cytologic staining; and (4) formalin-fixed, paraffin-embedded (FFPE) cell block processing on nucleic acid quality and quantity as determined by fragment analyzer, Qubit analysis (Thermo Fisher Scientific), and quantitative polymerase chain reaction-based analysis on the Idylla platform (Biocartis).

Results

Alcohol-based collection media (CytoRich Red [Thermo Fisher Scientific] and EtOH95%) displayed high DNA and RNA preservation capa

SUBMITTER: Sorber L 

PROVIDER: S-EPMC8824667 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature