Advances in enzyme-mediated proximity labeling and its potential for plant research.

ABSTRACT: Cellular processes rely on the intimate interplay of different molecules, including DNA, RNA, proteins, and metabolites. Obtaining and integrating data on their abundance and dynamics at high temporal and spatial resolution are essential for our understanding of plant growth and development. In the past decade, enzymatic proximity labeling (PL) has emerged as a powerful tool to study local protein and nucleotide ensembles, discover protein-protein and protein-nucleotide interactions, and resolve questions about protein localization and membrane topology. An ever-growing number and continuous improvement of enzymes and methods keep broadening the spectrum of possible applications for PL and make it more accessible to different organisms, including plants. While initial PL experiments in pla

SUBMITTER: Mair A 

PROVIDER: S-EPMC8825456 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature