Monitoring Distribution Dynamics of EV RNA Cargo Within Recipient Monocytes and Macrophages.

ABSTRACT: Extracellular vesicles (EVs) are produced by across almost all the living kingdoms and play a crucial role in cell-cell communication processes. EVs are especially important for pathogens, as Plasmodium falciparum (Pf) parasite, the leading causing species in human malaria. Malaria parasites are able to modulate the host immune response from a distance via delivering diverse cargo components inside the EVs, such as proteins and nucleic acids. We have previously shown that imaging flow cytometry (IFC) can be effectively used to monitor the uptake of different cargo components of malaria derived EVs by host human monocytes. Here, we take this approach one step further and demonstrate that we can directly investigate the dynamics of the cargo distribution pattern over time by mo

SUBMITTER: Alfandari D 

PROVIDER: S-EPMC8825493 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature