Network pharmacology analysis and experimental study strategy reveals the potential mechanism of puerarin against rotavirus.

ABSTRACT:

Background

This study aimed to explore the underlying mechanism of puerarin against rotavirus (RV), based on network pharmacology analysis and experimental study in vitro.

Methods

The cytopathic effect inhibition assay with different concentrations of puerarin at different times of infection in vitro was applied to evaluate the effect of puerarin against human RV G1P[8] Wa strain (HRV Wa). Subsequently, the potential targets of puerarin and RV-related genes were obtained from online databases. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the major target genes were also performed. Furthermore, the major targets and signaling pathway related to RV infection were verified at the molecular level via Western blot, quantitative real-

SUBMITTER: Chen T 

PROVIDER: S-EPMC8825547 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature