The decreased expression of miR-429 in plasma exosomes after spinal cord injury inhibits neuronal apoptosis by mediating the PTEN/PI3K/Akt pathway.

ABSTRACT:

Background

Neuronal apoptosis after spinal cord injury (SCI) leads to sensorial and motorial dysfunction. Exosomes are vesicles that contain many cellular components, including microRNA, and the role of miR-429 in plasma exosomes in this process after SCI requires further investigation.

Methods

The New York University impactor was used to create a rat model of SCI. We used SH-SY5Y cells to construct a neuronal apoptotic cell model and extracted plasma exosomes from rats in a stimulation. A miR-429 mimic and inhibitor were transfected, and the apoptosis-related indicators of the SH-SY5Y cells were detected by using western blot, cell-counting kit-8 and immunofluorescence. The possible targets of miR-429 were examined to verify the pathway of action. We then used the dual-luci

SUBMITTER: Huang J 

PROVIDER: S-EPMC8825548 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature