Gα₁₂ overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated miR-15a and ALOX12 dysregulation.

ABSTRACT: Rationale: Liver injury must be further characterized to identify novel therapeutic approaches. Endoplasmic reticulum (ER) stress may cause hepatocyte death. Gα₁₂ affects cell viability and its expression varies depending on physiological conditions. This study investigated whether hepatocyte-specific Gα₁₂ overexpression affects acute liver injury, and if so, what the underlying mechanisms and treatment strategies are. Methods: All experiments were performed using human liver, hepatocytes, and toxicant injury models with Gna12 KO and/or hepatocyte-specific Gα₁₂ overexpression. RNA-sequencing, immunoblotting, immunohistochemistry, reporter assays, and mutation assays were conducted. Results: Hepatic Gα₁₂ was overexpress

SUBMITTER: Tak J 

PROVIDER: S-EPMC8825599 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature