Binary Toxin Expression by Clostridioides difficile Is Associated With Worse Disease.

ABSTRACT:

Background

The incidence of Clostridioides difficile infection (CDI) has increased over the past 2 decades and is considered an urgent threat by the Centers for Disease Control and Prevention. Hypervirulent strains such as ribotype 027, which possess genes for the additional toxin C. difficile binary toxin (CDT), are contributing to increased morbidity and mortality.

Methods

We retrospectively tested stool from 215 CDI patients for CDT by enzyme-linked immunosorbent assay (ELISA). Stratifying patients by CDT status, we assessed if disease severity and clinical outcomes correlated with CDT positivity. Additionally, we completed quantitative PCR (PCR) DNA extracted from patient stool to detect cdtB gene. Lastly, we performed 16 S rRNA gene sequencing to examine i

SUBMITTER: Young MK 

PROVIDER: S-EPMC8825761 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature