Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production in Trichoderma reesei.

ABSTRACT: The filamentous fungus Trichoderma reesei secretes large quantities of cellulases and hemicellulases that have found wide applications in industry. Compared with extensive studies on the mechanism controlling cellulase gene expression, less is known about the regulatory mechanism behind xylanase gene expression. Herein, several putative sugar transporter encoding genes that showed significant upregulation on xylan were identified in T. reesei. Deletion of one such gene, gat1, resulted in increased xylanase production but hardly affected cellulase induction. Further analyses demonstrated that deletion of gat1 markedly increased XYNI production at the transcriptional level and only exerted a minor effect on XYNII synthesis. In contrast, overexpressing ga

SUBMITTER: Xu W 

PROVIDER: S-EPMC8825865 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature