Inositol 1,4,5-trisphosphate receptor - reactive oxygen signaling domain regulates excitation-contraction coupling in atrial myocytes.

ABSTRACT: The inositol 1,4,5-trisphosphate receptor (InsP₃R) is up-regulated in patients with atrial fibrillation (AF) and InsP₃-induced Ca²⁺ release (IICR) is linked to pro-arrhythmic spontaneous Ca²⁺ release events. Nevertheless, knowledge of the physiological relevance and regulation of InsP₃Rs in atrial muscle is still limited. We hypothesize that InsP₃R and NADPH oxidase 2 (NOX2) form a functional signaling domain where NOX2 derived reactive oxygen species (ROS) regulate InsP₃R agonist affinity and thereby Ca²⁺ release. To quantitate the contribution of IICR to atrial excitation-contraction coupling (ECC) atrial myocytes (AMs) were isolated from wild type and NOX2 deficient (Nox2^-/-) mice and chan

SUBMITTER: Varma D 

PROVIDER: S-EPMC8826595 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature