Site-specific DNA functionalization through the tetrazene-forming reaction in ionic liquids.

ABSTRACT: Development of multiple chemical tools for deoxyribonucleic acid (DNA) labeling has facilitated wide use of their functionalized conjugates, but significant practical and methodological challenges remain to achievement of site-specific chemical modification of the biomacromolecule. As covalent labeling processes are more challenging in aqueous solution, use of nonaqueous, biomolecule-compatible solvents such as an ionic liquid consisting of a salt with organic molecule architecture, could be remarkably helpful in this connection. Herein, we demonstrate site-specific chemical modification of unprotected DNAs through a tetrazene-forming amine-azide coupling reaction using an ionic liquid. This ionic liquid-enhanced reaction process has good functional group tolerance and precise chemoselecti

SUBMITTER: Ishizawa S 

PROVIDER: S-EPMC8826848 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature