The Combined Expression of the Nonstructural Protein NS1 and the N-Terminal Half of NS2 (NS2_1-180) by ChAdOx1 and MVA Confers Protection against Clinical Disease in Sheep upon Bluetongue Virus Challenge.

ABSTRACT: Bluetongue, caused by bluetongue virus (BTV), is a widespread arthropod-borne disease of ruminants that entails a recurrent threat to the primary sector of developed and developing countries. In this work, we report modified vaccinia virus Ankara (MVA) and ChAdOx1-vectored vaccines designed to simultaneously express the immunogenic NS1 protein and/or NS2-Nt, the N-terminal half of protein NS2 (NS2_1-180). A single dose of MVA or ChAdOx1 expressing NS1-NS2-Nt improved the protection conferred by NS1 alone in IFNAR(-/-) mice. Moreover, mice immunized with ChAdOx1/MVA-NS1, ChAdOx1/MVA-NS2-Nt, or ChAdOx1/MVA-NS1-NS2-Nt developed strong cytotoxic CD8⁺ T-cell responses against NS1, NS2-Nt, or both proteins and were fully protected against a lethal infection with BTV serotype

SUBMITTER: Utrilla-Trigo S 

PROVIDER: S-EPMC8826911 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature