Trapping and structural characterisation of a covalent intermediate in vitamin B₆ biosynthesis catalysed by the Pdx1 PLP synthase.

ABSTRACT: The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I₃₃₃ intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.

SUBMITTER: Rodrigues MJ 

PROVIDER: S-EPMC8827014 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature