An improved repertoire of splicing variants and their potential roles in Arabidopsis photomorphogenic development.

ABSTRACT:

Background

Light switches on the photomorphogenic development of young plant seedlings, allowing young seedlings to acquire photosynthetic capacities and gain survival fitness. Light regulates gene expression at all levels of the central dogma, including alternative splicing (AS) during the photomorphogenic development. However, accurate determination of full-length (FL) splicing variants has been greatly hampered by short-read RNA sequencing technologies.

Result

In this study, we adopt PacBio isoform sequencing (Iso-seq) to overcome the limitation of the short-read RNA-seq technologies. Normalized cDNA libraries used for Iso-seq allows for comprehensive and effective identification of FL AS variants. Our analyses reveal more than 30,000 splicing variant models from approxim

SUBMITTER: Huang CK 

PROVIDER: S-EPMC8827149 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature