FluoRNT: A robust, efficient assay for the detection of neutralising antibodies against yellow fever virus 17D.

ABSTRACT: There is an urgent need for better diagnostic and analytical methods for vaccine research and infection control in virology. This has been highlighted by recently emerging viral epidemics and pandemics (Zika, SARS-CoV-2), and recurring viral outbreaks like the yellow fever outbreaks in Angola and the Democratic Republic of Congo (2016) and in Brazil (2016-2018). Current assays to determine neutralising activity against viral infections in sera are costly in time and equipment and suffer from high variability. Therefore, both basic infection research and diagnostic population screenings would benefit from improved methods to determine virus-neutralising activity in patient samples. Here we describe a robust, objective, and scalable Fluorescence Reduction Neutralisation Test (FluoRNT) for ye

SUBMITTER: Scheck MK 

PROVIDER: S-EPMC8827462 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature