Ca⁺ activity maps of astrocytes tagged by axoastrocytic AAV transfer.

ABSTRACT: Astrocytes exhibit localized Ca²⁺ microdomain (MD) activity thought to be actively involved in information processing in the brain. However, functional organization of Ca²⁺ MDs in space and time in relationship to behavior and neuronal activity is poorly understood. Here, we first show that adeno-associated virus (AAV) particles transfer anterogradely from axons to astrocytes. Then, we use this axoastrocytic AAV transfer to express genetically encoded Ca²⁺ indicators at high-contrast circuit specifically. In combination with two-photon microscopy and unbiased, event-based analysis, we investigated cortical astrocytes embedded in the vibrissal thalamocortical circuit. We found a wide range of Ca²⁺ MD signals, some of which were ultrafast (≤300 ms)

SUBMITTER: Georgiou L 

PROVIDER: S-EPMC8827655 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature