Recapitulation of dyssynchrony-associated contractile impairment in asymmetrically paced engineered heart tissue.

ABSTRACT:

Background

One third of heart failure patients exhibit dyssynchronized electromechanical activity of the heart (evidenced by a broad QRS-complex). Cardiac resynchronization therapy (CRT) in the form of biventricular pacing improves cardiac output and clinical outcome of responding patients. Technically demanding and laborious large animal models have been developed to better predict responders of CRT and to investigate molecular mechanisms of dyssynchrony and CRT. The aim of this study was to establish a first humanized in vitro model of dyssynchrony and CRT.

Methods

Cardiomyocytes were differentiated from human induced pluripotent stem cells and cast into a fibrin matrix to produce engineered heart tissue (EHT). EHTs were either field stimulated in their entirety (symmetric

SUBMITTER: Stenzig J 

PROVIDER: S-EPMC8828044 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature