RNA binding to human METTL3-METTL14 restricts N⁶-deoxyadenosine methylation of DNA in vitro.

ABSTRACT: Methyltransferase like-3 (METTL3) and METTL14 complex transfers a methyl group from S-adenosyl-L-methionine to N⁶ amino group of adenosine bases in RNA (m⁶A) and DNA (m⁶dA). Emerging evidence highlights a role of METTL3-METTL14 in the chromatin context, especially in processes where DNA and RNA are held in close proximity. However, a mechanistic framework about specificity for substrate RNA/DNA and their interrelationship remain unclear. By systematically studying methylation activity and binding affinity to a number of DNA and RNA oligos with different propensities to form inter- or intra-molecular duplexes or single-stranded molecules in vitro, we uncover an inverse relationship for substrate binding and methylation and show that METTL3-METT

SUBMITTER: Qi S 

PROVIDER: S-EPMC8828048 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature