Toll-7 promotes tumour growth and invasion in Drosophila.

ABSTRACT:

Objectives

Drosophila melanogaster has become an excellent model organism to explore the genetic mechanisms underlying tumour progression. Here, by using well-established Drosophila tumour models, we identified Toll-7 as a novel regulator of tumour growth and invasion.

Materials and methods

Transgenic flies and genetic epistasis analysis were used. All flies were raised on a standard cornmeal and agar medium at 25°C unless otherwise indicated. Immunostaining and RT-qPCR were performed by standard procedures. Images were taken by OLYMPUS BX51 microscope and Zeiss LSM 880 confocal microscope. Adobe Photoshop 2020 and Zeiss Zen were used to analyse the images. All results were presented in Scatter plots or Column bar graphs created by GraphPad Prism 8.0.

Results

Loss of

SUBMITTER: Ding X 

PROVIDER: S-EPMC8828261 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature