A long-read amplicon approach to scaling up the metabarcoding of lichen herbarium specimens.

ABSTRACT: Reference sequence databases are critical to the accurate detection and identification of fungi in the environment. As repositories of large numbers of well-curated specimens, herbaria and fungal culture collections have the material resources to generate sequence data for large number of taxa, and could therefore allow filling taxonomic gaps often present in reference sequence databases. Financial resources to do that are however often lacking, so that recent efforts have focused on decreasing sequencing cost by increasing the number of multiplexed samples per sequencing run while maintaining high sequence quality. Following a previous study that aimed at decreasing sequencing cost for lichen specimens by generating fungal ITS barcodes for 96 specimens using PacBio amplicon sequencing, we

SUBMITTER: Gueidan C 

PROVIDER: S-EPMC8828592 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature