Human neural tube morphogenesis in vitro by geometric constraints.

ABSTRACT: Understanding human organ formation is a scientific challenge with far-reaching medical implications^1,2. Three-dimensional stem-cell cultures have provided insights into human cell differentiation^3,4. However, current approaches use scaffold-free stem-cell aggregates, which develop non-reproducible tissue shapes and variable cell-fate patterns. This limits their capacity to recapitulate organ formation. Here we present a chip-based culture system that enables self-organization of micropatterned stem cells into precise three-dimensional cell-fate patterns and organ shapes. We use this system to recreate neural tube folding from human stem cells in a dish. Upon neural induction^5,6, neural ectoderm folds into a millimetre-long neural tube covered with non-neur

SUBMITTER: Karzbrun E 

PROVIDER: S-EPMC8828633 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature