Response surface methodological optimization of L-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1.

ABSTRACT:

Background

This study targets the enhanced production of L-asparaginase, an antitumor enzyme by Acinetobacter baumannii ZAS1. This organism is an endophyte isolated from the medicinal plant Annona muricata. Plackett-Burman design (PBD) and central composite design (CCD) were used for statistical optimization of media components.

Results

The organism exhibited 18.85 ± 0.2 U/mL enzyme activities in unoptimized media. Eight variables: L-asparagine, peptone, glucose, lactose, yeast extract, NaCl, MgSO₄, and Na₂HPO₄ were screened by PBD. Among them, only four factors-L-asparagine, peptone, glucose, and Na₂HPO₄-were found to affect enzyme production significantly (p < 0.05). Furthermore, the best possible concentrations and in

SUBMITTER: Abhini KN 

PROVIDER: S-EPMC8828825 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature