Primary glia cells from bank vole propagate multiple rodent-adapted scrapie prions.

ABSTRACT: Since the beginning prion research has been largely dependent on animal models for deciphering the disease, drug development or prion detection and quantification. Thereby, ethical as well as cost and labour-saving aspects call for alternatives in vitro. Cell models can replace or at least complement animal studies, but their number is still limited and the application usually restricted to certain strains and host species due to often strong transmission barriers. Bank voles promise to be an exception as they or materials prepared from them are uniquely susceptible to prions from various species in vivo, in vitro and in cell-free applications. Here we present a mainly astrocyte-based primary glia cell assay from bank vole, which is infectible with scrapie strains from bank vole, mouse and

SUBMITTER: Schwenke KA 

PROVIDER: S-EPMC8828835 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature