The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction.

ABSTRACT: The Y-chromosomal short tandem repeat (Y-STR) is an effective forensic tool in familial searches and patrilineal relationship evaluation. However, currently available Y-STR panels often lack sufficient discriminatory power to resolve genetic relationships between distant relatives or within patrilocal populations. This study aims to establish a novel Y-STR amplification system for forensic casework analysis and database construction, which contains 44 slowly and moderately mutating and one rapidly mutating Y-STR. The validation of the assay was conducted following the recommendations of SWGDAM developmental validation guidelines. Different types of casework samples were tested and reliable profiles were obtained. Furthermore, we genotyped and analyzed 141 unrelated Han Chinese male samples

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC8829124 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature