PiggyBac Transposon-Mediated CD19 Chimeric Antigen Receptor-T Cells Derived From CD45RA-Positive Peripheral Blood Mononuclear Cells Possess Potent and Sustained Antileukemic Function.

ABSTRACT: The quality of chimeric antigen receptor (CAR)-T cell products, namely, memory and exhaustion markers, affects the long-term functionality of CAR-T cells. We previously reported that piggyBac (PB) transposon-mediated CD19 CAR-T cells exhibit a memory-rich phenotype that is characterized by the high proportion of CD45RA⁺/C-C chemokine receptor type 7 (CCR7)⁺ T-cell fraction. To further investigate the favorable phenotype of PB-CD19 CAR-T cells, we generated PB-CD19 CAR-T cells from CD45RA⁺ and CD45RA^- peripheral blood mononuclear cells (PBMCs) (RA⁺ CAR and RA^- CAR, respectively), and compared their phenotypes and antitumor activity. RA⁺ CAR-T cells showed better transient gene transfer efficiency 24 h after

SUBMITTER: Suematsu M 

PROVIDER: S-EPMC8829551 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature