Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the Bacillus subtilis plasmid pLS20.

ABSTRACT: Bacterial conjugation is an important route for horizontal gene transfer. The initial step in this process involves a macromolecular protein-DNA complex called the relaxosome, which in plasmids consists of the origin of transfer (oriT) and several proteins that prepare the transfer. The relaxosome protein named relaxase introduces a nick in one of the strands of the oriT to initiate the process. Additional relaxosome proteins can exist. Recently, several relaxosome proteins encoded on the Bacillus subtilis plasmid pLS20 were identified, including the relaxase, named Rel_pLS20, and two auxiliary DNA-binding factors, named Aux1_pLS20 and Aux2_pLS20. Here, we extend this characterization in order to define their function. We present the low-r

SUBMITTER: Crespo I 

PROVIDER: S-EPMC8829557 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature