Preservation of the naive features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix.

ABSTRACT: The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-s

SUBMITTER: Pereira AR 

PROVIDER: S-EPMC8829705 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature